Serial Dilution System

Dilucup – a new way of handling serial dilutions of bacteriological samples.
LabRobot Products AB has developed a new concept that makes serial
dilutions easier, faster and more cost efficient.
Today, the most normal way of diluting a bacteriological sample in 1:10,
1:100, 1:1000 etc., is to prepare own test tubes. These tubes
are filled with a diluting media, for example pepton + salt. The tubes are
sterilized by using an autoclave and then stored in a refrigerator to
prevent bacterial growth. After used, the tubes are cleaned in
order to be ready to be filled again. To prepare and fill test tubes for
serial dilution is both time consuming and expensive.

The Dilucup Concept
Dilucup is a unique prefilled container with a sterile diluting media used in combination with a special shaker, the Dilushaker. Compared to normal dilution tubes the time save is at least 10 seconds/dilution step when using Dilucup. The Dilucup is manufactured under controlled conditions and sterilized by gamma radiation. This guarantees a high quality standard. All Dilucups are supplied with a quality certificate.


Dilucup



The Dilucup is delivered in blisters of rows of either 3 or 6 containers, each filled with 9 ml diluting media. It is easy to separate the number of Dilucups needed. The Dilucups are placed on the tray of the Dilushaker, (there are two standard tray models; one for 21 and another for 42 Dilucups). The Dilucups are opened by tearing off the film covering the holes in the lids. When the Dilushaker is turned on, the liquid in all Dilucups rotates. A sample of 1 ml is added to the first cup and is instantly mixed with the media. After changing the pipette tip, 1 ml of sample is withdrawn from the first cup and added to the second. With the Dilushaker operating the process is repeated until the required dilution factor is achieved. The Dilucup is today patented in Europe plus USA and has pending patents in other countries.


The advantages with the Dilucup:
• Cost saving
Calculating all costs for producing own dilution
tubes the cost saving by using Dilucup will be
considerable.
• Time saving
The time it takes to make a dilution using Dilucup
is more or less dependent on how fast one can
change pipette tips – no more moving of tubes
back and forth to the vortex mixer. The calculated
timesaving is approximately 10 seconds/tube compared
to the traditional method.
• Preventing working injuries
Holding the tube while it shakes on the Vortex mixer and moving it back and forth can cause severe working injuries.
• Increased quality
The dilution process is standardized and consistent, (same time and speed for all dilutions).
By using high precision filling machines, gamma sterilization, and an accurate quality control, a higher quality product can be guarantied.
The Dilucups are supplied with a quality certifcate.
• Easy to store and long durability
The sealed Dilucup boxes can be stored at normal room temperature during 12 months.
When opened, the package with the dilucups will no longer have the
original expiring date and must therefore be kept in a refrigerator room
and for no longer than 2 months. The original volume of media in the
cups will change if the package is kept open and at normal room temperature.



Dilucup Leaflet